2017-8-11 16:0:32025-05-21 08:14:06
locus
BSU09370
BSU_09370
geneLength
1464
1467
outlinks
bsu
BSU09370
BSU_09370
The protein
Catalyzed reaction/ biological activity
cleaves the peptide bond between D-Glu (position 2 in the peptioglycan peptide) and m-diamino pimelic acid (position 3) [Pubmed|18266855]
cleaves the peptide bond between D-Glu (position 2 in the peptioglycan peptide) and m-diamino pimelic acid (position 3) [Pubmed|18266855]
degradation of gamma-polyglutamic acid [pubmed|29458655]
The protein
Protein family
nlpC/p60 family (according to Swiss-Prot)
[SW|Peptidase C40 family] (according to UniProt)
The protein
Paralogous protein(s)
the C-terminal D,L-endopeptidase domains of [[protein|LytE]], [[protein|LytF]], [[protein|CwlS]], and [[protein|CwlO]] exhibit strong sequence similarity
[[this]]
The protein
[SW|Domains]
contains five N-acetylglucosamine-polymer-binding [SW|LysM domain]s [Pubmed|18430080]
C-terminal D,L-endopeptidase domain [Pubmed|22139507]
contains five N-acetylglucosamine-polymer-binding [SW|LysM domain]s [Pubmed|18430080]
C-terminal D,L-endopeptidase domain ([SW|NlpC/P60 domain]) [pubmed|29458655,22139507]
5 [SW|LysM domain]s (aa 27-70, aa 92-135, aa 174-217, aa 240-283, aa 307-350) (according to UniProt)
Biological materials
Mutant
1A791 ( ''lytF''::''spec''), [Pubmed|10206711], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A791&Search=1A791 BGSC]
1A792 ( ''lytF''::''spec''), [Pubmed|1588906], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A792&Search=1A792 BGSC]
1A791 ( ''lytF''::''spec''), [Pubmed|10206711], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A791&Search=1A791 BGSC]
1A792 ( ''lytF''::''spec''), [Pubmed|1588906], available at [http://pasture.asc.ohio-state.edu/BGSC/getdetail.cfm?bgscid=1A792&Search=1A792 BGSC]
BKE09370 (Δ[[gene|lytF]]::erm trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKE09370 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTTAGCTCTCCTTTTTC, downstream forward: _UP4_TAAAAACAGAAACTGTGCGG
BKK09370 (Δ[[gene|lytF]]::kan trpC2) available at [http://www.bgsc.org/getdetail.php?bgscid=BKK09370 BGSC], [Pubmed|28189581], upstream reverse: _UP1_CATCTTAGCTCTCCTTTTTC, downstream forward: _UP4_TAAAAACAGAAACTGTGCGG
References
Original publications
The protein
Effectors of protein activity
both enzymatic activities are inhibited by interaction with [[protein|IseA]] [pubmed|29458655]
The protein
Structure
[PDB|4XCM] (from Thermus thermophilus, 31% identity) [pubmed|25760608]